date |
time |
type of activity |
person in charge |
subject |
September 5th |
morning |
Arrival by boat; check-in, |
||
Sunday |
noon |
security breefing,WSBS orientation tour introduction to WSBS to to molecular zoology school |
||
afternoon |
Zhadan, |
Littoral zone - lecture, sauna |
||
night |
Tzetlin |
Benthic fauna of arctic seas(with movies), welcome party |
||
September 6th |
morning |
excursion |
Zhadan |
Littoral zone excursion. Collection samples for identification and DNA extraction. |
Monday |
afternoon |
lab |
Zhadan, |
Sample processing, species Identification |
evening lect. |
TBA |
|||
September 7th |
mornung |
lecture |
Mugue |
DNA extraction techniques |
Tuesday |
mornung |
lab |
Neretina, Azhikina |
DNA extraction from Dulichia by NucleoS (quick protocol) |
afternoon |
lab |
Neretina, Azhikina |
PCR from prepared DNA samples (new Dulichia microsats primers) |
|
evening lect. |
lecture |
TBA |
||
September 8th |
morning |
lecture |
Tzetlin or Zhadan |
Methods in marine biology. Different types of instruments for faunistic survey. Breefing on personal security on board of R/V |
Wednesday |
afternoon |
excursion |
Tzetlin, Mugue |
Benthic survey -trawling and/or grab sampling on R/V "Belomor |
evening |
lab |
Zhadan, Mordashova, Kolbasova |
Processing of benthic samples from "Belomor" trawling and grab sampling, acquaintance with key benthic species. |
|
evening lect. |
TBA |
|||
September 9th |
morn.lecture |
morning |
Mugue |
Introduction to PCR, overview of methods based on PCR |
Thursday |
morning lab |
lab |
Azhikina, Lebedev |
DNA extraction from different benthic specimens (Wizard) |
noon lab |
lab |
Azhikina, Lebedev |
PCR with different (barcoding) primers, PCR optimization, agarose electrophoresis, purification for sequencing, ligation setup |
|
evening lect. |
Mugue |
Sticklebacks as a model for speciation study |
||
September 10th |
morning |
excursion |
Neretina, Mugue |
excursion to Ershov Lake, stickleback sampling |
Friday |
noon lab |
evening |
Stremovskiy, Suslova |
DNA extraction from agarose gel; purification for sequencing purpose; purification for ligation purpose; ligation setup |
night lab |
Stremovskiy, Suslova |
DNA extraction from Stickleback by salt method (set up overnight lysis ) |
||
September 11th |
morning |
lecture |
Mugue |
Assesment of genetic polymorphism in natural populations - SNP, microsatellites, AFLP etc. |
Saturday |
morning |
lab |
Neretina, Stremovskiy |
DNA extraction from Stickleback by salt method (cont. ) |
afternoon |
evening |
Neretina, Stremovskiy |
Eda1 polymorphism analysis (along with banya) |
|
evening lect. |
TBA |
|||
September 12th |
whole day |
whole day |
excursion to Kasyan Island |
|
Sunday |
||||
September 13th |
morn.lecture |
Matz |
introduction to qPCR |
|
Monday |
morning |
lab |
Matz , Neretina |
RNA extraction; electrophoresis, DNAse treatment |
afternoon |
lab |
Matz , Neretina |
First cDNA strand synthesis; setting up stickleback experiment (fresh and saltwater tanks) |
|
evening lect. |
Matz |
Studying local adaptation in corals using qPCR (Matz) |
||
September 14th |
morn.lecture |
lecture |
Matz |
qPCR: relative quantification theory |
Tuesday |
morning lab |
lab |
Matz , Neretina |
Testing qPCR primers in dilution series |
afternoon lab |
lab |
Matz , Neretina |
RNA extraction from the experiment, DNAse, cDNA synthesis |
|
evening lect. |
comp.lab |
Matz |
Analysis of primer efficiency data using R |
|
September 15th |
morn.lecture |
lecture |
Matz |
no lecture, straight to lab |
Wednesday |
morning lab |
lab |
Matz , Neretina |
qPCR of experimental cDNAs (5 genes, 16 samples, replicate 1) |
noon lab |
lab |
Matz , Neretina |
qPCR of experimental cDNAs (5 genes, 16 samples, replicate 2) |
|
evening |
comp.lab |
Matz |
qPCR data analysis using R |
|
September 16th |
morning |
|||
Thursday |
afternoon |
lab |
PCR optimization with Dulichia microsats |
|
evening |
PAAG electrophoresis |
|||
September 17th |
morning |
excursion |
Zhadan, Tzetlin |
Benthic survey -trawling and/or grab sampling on R/V "Belomor at deep see locations |
Friday |
whole day |
lab |
Zhadan, Mordashova, Kolbasova |
Survey of White Sea marine fauna (cont.) Species identification, labling, maintainance of museum collectiomns |
evening lect. |
TBA or student's colloquium |
|||
September 18th |
morn.lecture |
lecture |
Mugue |
metods in phylogenetic analysis (parsymony, ML, bayesian) |
Saturday |
morning lab |
computer lab |
Mugue |
metods in phylogenetic analysis (parsymony, ML, bayesian) |
evening |
sauna; farewell party |
|||
September 19th |
morning |
morning |
all |
Departure by boat to Poyakonda |
Sunday |
page updated: October 23, 2010 3:31 PM
